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pcat basic vector  (Thermo Fisher)


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    Thermo Fisher pcat basic vector
    Pcat Basic Vector, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/pcat+basic/1KB+PLUS+DNA+LADDER/pm31994826-150-4-19
    Average 99 stars, based on 1 article reviews
    pcat basic vector - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Sequencing:

    Article Title: Down-regulation of hepatic lipase expression by elevation of cAMP in human hepatoma but not adrenocortical cells.
    Article Snippet: .. All inserts in pGL3-Basic and pCAT-Basic were verified y cycle sequencing using the Thermo Sequenase dye terminator kit (Amersham) nd the ABI 377 sequencer. .. Plasmid DNAs were isolated with the Wizard Midiprep ystem (Promega).

    Plasmid Preparation:

    Article Title: Regulatory SNP rs5743417 impairs constitutive expression of human β-defensin 1 and has high frequency in Africans and Afro-Americans.
    Article Snippet: 1Interinstitutional Posgrade in Science and Technology (PICYT), Research Center of Technology and Design Assistance of Jalisco State, (CIATEJ A.C.), Guadalajara, Mexico 2Laboratory of Regulatory SNPs, Personalized Medicine National Laboratory (LAMPER), Pharmaceutical and Medical Biotechnology, Central Unit, CIATEJ A.C., National Council of Science and Technology (CONACYT), Guadalajara, Mexico 3Faculty of Chemical and Biological Sciences, Autonomous University of Sinaloa, Culiacan, Mexico 4Faculty of Biology, Autonomous University of Sinaloa, Culiacan, Mexico 5Genomic Medicine Center, Dr. Bernardo J. Gastélum Primary Care Hospital, Sinaloa Health Ministry, Culiacan, Mexico 6Laboratory of Pharmacogenomics and Preventive Medicine, Personalized Medicine National Laboratory (LAMPER), Pharmaceutical and Medical Biotechnology, Central Unit, CIATEJ A.C., CONACYT, Guadalajara, Mexico 7Scripps Research Translational Institute, La Jolla, CA, USA 8Integrative Structural and Computational Biology, Scripps Research Institute, La Jolla, CA, USA



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    Image Search Results


    The CAT expression levels under the complete or divided promoters in opposite directions in uninfected, or rMd5-infected CEFs transfected with a set of CAT reporter plasmids

    Journal: Virology Journal

    Article Title: The construction and characterization of the bi-directional promoter between pp38 gene and 1.8-kb mRNA transcripts of Marek's disease viruses

    doi: 10.1186/1743-422X-6-212

    Figure Lengend Snippet: The CAT expression levels under the complete or divided promoters in opposite directions in uninfected, or rMd5-infected CEFs transfected with a set of CAT reporter plasmids

    Article Snippet: Lipofectamine™ was purchased from Invitrogen (Beijing, China); plasmid purification Mini Kit was from Qiagen (Shanghai, China); pCAT-Basic vector was from Promega (Beijing, China); CAT ELISA detection Kit was from Roche (Shanghai, China); SPF chicken embryos were from SFAFAS Company (Jinan, China).

    Techniques: Expressing, Transfection

    Relative enhancer activities of fragments of HcTnC intron 1. pHcTnC4500CAT linked to various intron fragments (see Fig. 1) were transfected into C2 myogenic cells and transient CAT activity measured as described in Materials and Methods. All intron constructs carry the HcTnC4500 full promoter cloned into pCAT Basic (Promega) (12). Relative CAT expression was compared to the wild-type reference construct pHcTnC4500CAT/Il-500-S defined at 100% expression. The figure displays the mean values and the SD based on observations for four or more independent experiments. The intronless-promoterless pCAT Basic (pOCAT) served as a negative control.

    Journal: Gene Expression

    Article Title: The Myogenic Regulatory Circuit That Controls Cardiac/Slow Twitch Troponin C Gene Transcription in Skeletal Muscle Involves E-box, MEF-2, and MEF-3 Motifs

    doi:

    Figure Lengend Snippet: Relative enhancer activities of fragments of HcTnC intron 1. pHcTnC4500CAT linked to various intron fragments (see Fig. 1) were transfected into C2 myogenic cells and transient CAT activity measured as described in Materials and Methods. All intron constructs carry the HcTnC4500 full promoter cloned into pCAT Basic (Promega) (12). Relative CAT expression was compared to the wild-type reference construct pHcTnC4500CAT/Il-500-S defined at 100% expression. The figure displays the mean values and the SD based on observations for four or more independent experiments. The intronless-promoterless pCAT Basic (pOCAT) served as a negative control.

    Article Snippet: All intron constructs carry the HcTnC4500 full promoter cloned into pCAT Basic (Promega) ( 12 ).

    Techniques: Transfection, Activity Assay, Construct, Clone Assay, Expressing, Negative Control